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Fig. 2 | BMC Cancer

Fig. 2

From: Identification of an easy to use 3D culture model to investigate invasion and anticancer drug response in chondrosarcomas

Fig. 2

3D culture in alginate favors expression of collagen type II by chondrosarcomas. Chondrosarcomas were cultured in monolayer, or embedded in alginate for 1, 2 or 3 weeks. Then, alginate was dissolved and RNA extracted. Collagen type II and type I, and COMP mRNA levels were assayed by real-time RT-PCR after normalization to RPL13 signal. Values are the mean ± SEM of triplicate experiments

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