Fig. 2From: Identification of an easy to use 3D culture model to investigate invasion and anticancer drug response in chondrosarcomas3D culture in alginate favors expression of collagen type II by chondrosarcomas. Chondrosarcomas were cultured in monolayer, or embedded in alginate for 1, 2 or 3 weeks. Then, alginate was dissolved and RNA extracted. Collagen type II and type I, and COMP mRNA levels were assayed by real-time RT-PCR after normalization to RPL13 signal. Values are the mean ± SEM of triplicate experimentsBack to article page