Arrb1 is down regulated in SHH-MB CSCs.
a miR-326 intragenic localization in the first intron of the Arrb1 gene on murine chromosome 7 and human chromosome 11. b WB (Left panel) of Arrb1 and Nanog levels in SM (CSCs) vs pre-oncosphere cell population (T0). mRNA expression (Right panel) analysis of Arrb1 (Arrb1) levels MB cells grown in CSCs vs T0. Bar graphs represent mean ± S.D. from three independent experiments. *p < 0.05. c Arrb1 and Nanog expression levels in CSCs grown as oncospheres in SM and exposed to differentiation stimuli (DFM). LC: Actin. d Oncosphere forming assay (left panel) and bromodeoxyuridine (BrdU) uptake (right panel) in CSCs after ectopic expression of miR-326 and Arrb1. Data represent mean ± S.D. from five independent experiments. *p < 0.05