Fig. 4
From: Trop2 enhances invasion of thyroid cancer by inducing MMP2 through ERK and JNK pathways
Trop2 regulates the expression of MMP2. a Silencing of Trop2 in K1 and 8505C thyroid cancer cells resulted in down-regulation of MMP2 expression, but not MMP7, MMP9, MMP11, and MMP13 expression. The data are reported as mean ± SD of three independent experiments. *, P < 0.05. b Depletion of Trop2 in K1 and 8505C thyroid cancer cells resulted in inhibition of the MMP2 promoter reporter activity. The data are reported as mean ± SD of three independent experiments. *, P < 0.05. c Ectopic overexpression of Trop2 promotes the expression of MMP2 in FTC-133 thyroid cancer cells. The data are reported as mean ± SD of three independent experiments. *, P < 0.05. d Trop2 overexpression enhanced the MMP2 promoter reporter activity. The data are reported as mean ± SD of three independent experiments. *, P < 0.05. e Conditioned media were prepared by incubating vector-control or Trop2-knockdown cells in serum-free media for 24 h. MMP2 activities are analyzed by gelatin zymography. f Gelaltin zymography analysis of serum-free conditioned medium from FTC-133-vector, FTC-133-Trop2 cells