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Fig. 2 | BMC Cancer

Fig. 2

From: Trop2 enhances invasion of thyroid cancer by inducing MMP2 through ERK and JNK pathways

Fig. 2

Knockdown of endogenous Trop2 suppresses the invasion and migration of thyroid cancer cells. a Trop2 knockdown was achieved by introducing specific shRNAs in thyroid cancer cells. The protein levels of Trop2 in indicated cells were assessed by WB. α-tubulin was used as a loading control. b Silencing of Trop2 led to significant decrease of invasive capability of thyroid cancer cells. The indicated cells traveled through the membrane by Transwell invasion assay (coated with Matrigel) were stained with crystal violet and imaged using a microscope. c The histogram showed the invading cells per field. The data are reported as mean ± SD of three independent experiments. *, P < 0.05. d Representative images of Transwell migration assay (coated without Matrigel) results of indicated cells. e Diagram of Transwell migration assay of indicated cells. The data are reported as mean ± SD of three independent experiments. *, P < 0.05. f Wound healing analyses of indicated cells. Streaks were created with a tip, and the representative phase-contrast images of the extent of cell migration into the wounded area at 24 h are shown. Experiments were repeated for three times

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