Fig. 2

Loss of DIP2C induces changes in gene expression. Expression levels determined by RNA sequencing were verified by RT-qPCR for six upregulated and six downregulated genes in DIP2C ^−/− cells. White bars – RT-qPCR, black bars – RNA sequencing. Mean expression in two biological replicates of DIP2C ^−/− #1-1 and one DIP2C ^−/− #1-2 sample normalized to the mean of biological triplicates of parental RKO is shown for RT-qPCR data. All samples were run with technical triplicates. Error bars, SD. Mean log2 fold change for DIP2C ^−/− #1-1 and #1-2 relative parental RKO is shown for RNA sequencing data. DCLK1 was not detectable by RT-qPCR in DIP2C ^−/− cells although stably expressed in parental RKO and is therefore not included in the graph