Fig. 4

TAM reversed EMT by up-regulating miR-200c of mesenchymal TNBC cells. a, Comparison of miRNAs expression in MCF-7/ADR cells and MCF-7 cells was performed by microarray analysis. After normalization, differentially expressed (>2 fold) miRNAs were identified through Volcano Plot filtering. b, Relative expression of miR-200c in MCF-7 and MCF-7/ADR cells determined by microarray analysis. c, Relative expression of miR-200c in MCF-7 and MCF-7/ADR cells determined by realtime-PCR. d, Relative expression of miR-200c cells in MCF-7 and MCF-7/ADR cells treated with or without TAM for 48 h determined by realtime-PCR. miRNA expression were normalized to small nuclear ribonucleic acid (snRNA) RNU6B, and results were expressed as fold change relative to control (con). All experiments were repeated 3 times. Results were expressed as mean ± SD (n = 3). * P < 0.05