Fig. 8

UMI-77 disrupts the endogenous protein-protein interactions of MCL-1 with BAX and BAK. a, b, c and d KYSE150 (a, b) and KYSE510 (c, d) cells were starved in 0.1% FBS/RPMI 1640 medium overnight and then cultured without (DMSO) or with different concentrations of UMI-77 in 10% FBS/RPMI 1640 medium for 48 h. After treatment, attached and floating cells were harvested. Cells were lysed and immunoprecipitated with an anti-MCL-1 or a normal IgG antibody. The immune complexes and input were analyzed by immunoblotting with a BAX (a, c) or BAK (b, d) antibody