Fig. 3

Transient reduction of MCL-1 expression in KYSE150 and KYSE510 cells suppresses cell proliferation. a MCL-1 protein expression was reduced by siRNA in both KYSE150 and KYSE510 cells. Cells were transiently transfected with an Mcl-1 siRNA (si-MCL-1, 50 nM) or a control siRNA (si-Ctrl, 50 nM) for 48 h. Cell lysates were examined of MCL-1 level by Western blotting. β-actin was use as a loading control. b KYSE150 and KYSE510 cells were transiently transfected with an Mcl-1 siRNA (50 nM) or a control siRNA (50 nM) for 24 h. Cell viability was measured with the WST-1 assay at 48 h and 72 h after siRNA transfection. All experiments were performed in triplicate with at least two independent experiments. **p < 0.01, ***p < 0.001, significant difference compared with the si-Ctrl cells