Fig. 4
From: Inhibition of HDAC6 activity through interaction with RanBPM and its associated CTLH complex
RanBPM requires the LisH and CTLH domains to associate with α-tubulin. Hela RanBPM shRNA cells were transfected with the indicated HA-RanBPM deletion constructs. Cells were fixed and incubated with antibodies against HA (green) and α-tubulin (red). Colocalization of HA-RanBPM and α-tubulin was analyzed using Imaris software. Top, representative images where white signal represents the top 2% of HA-RanBPM and α-tubulin colocalization. Scale bar: 10 μm. Bottom, Quantification of the top 2% of pixels representing HA-RanBPM colocalized with α-tubulin. Data are representative of a minimum of 10 transfected cells from three experiments. Error bars represent SEM. P < 0.05 (*)