Fig. 1
From: Inhibition of HDAC6 activity through interaction with RanBPM and its associated CTLH complex
RanBPM association with HDAC6 is required for inhibition of HDAC6 deacetylase activity. Top, whole cell extracts from RanBPM shRNA Hela cells either left untransfected or transfected with HA-RanBPM-WT or HA-RanBPM-Δ360 were analyzed by western blot with the antibodies indicated. Bottom, quantification of relative amounts of acetylated α-tubulin was normalized to total α-tubulin levels. Relative amounts of acetylated α-tubulin in untransfected and HA-RanBPM-Δ360-transfected cells were normalized to WT. Results are averaged from three different experiments, with error bars indicating SEM. P < 0.05 (*)