Fig. 5
From: Regulation of early growth response 2 expression by secreted frizzled related protein 1
The effect of rSFRP1 treatment on M1 and M2 polarization in human mammary gland explant cultures. a Total RNA was isolated from explant cultures dervived from normal human breast tissue treated in the absence and presence of 1 μg/ml rSFRP1. a M2 polarization was evaluated by the mRNA expression of CD163 (left panal) and M1 polarization was evaluated by HLA-DRA mRNA expression (right panal). b Explant mammary gland sections were subjected to immunohistochemical analysis, stained for CD163 (left panel) or HLA-DRA (right panel) and images were captured at 100X. Representative pictures are displayed for tissues from each treatment group which was performed in triplicate samples. *p < 0.05 (significantly different from untreated mammary tissue using student’s t-test)