Fig. 2
From: Regulation of early growth response 2 expression by secreted frizzled related protein 1
The expression of EGR2 is regulated by TGF-β and MAPK signaling in human and mouse mammary epithelial cells with reduced SFRP1 expression. a TERT-pSUPER and TERT-siSFRP1 cells were treated with 10 μM LY364947 (left panel), or 5 μM U0126 (right panel), for 24 h and total RNA was isolated from three separate harvests for real-time PCR analysis of EGR2. b Mouse mammary epithelial cells were treated with 10 μM LY364947 (left panel), or 5 μM U0126 (right panel), for 24 h and total RNA was isolated from three separate harvests for real-time PCR analysis of Egr2. All real-time PCR results are from two separate experiments performed in triplicate and results were normalized to amplification of ACTB mRNA. Bars represent mean ± SEM and are expressed as fold change with respect to TERT-pSUPER cells or control MMECs. *p < 0.05, **p < 0.01 (significantly different from DMSO treated group using student’s t-test)