Fig. 4
In vivo bioluminescence of MDA-MB-231 in the orthotropic animal model. a 5 X 106 luciferase-expressed MDA-MB-231 cells were IP injected orthotropically into the mammary fat pad. The mice with PBS were the control group. All mice were fed a normal diet for two months. For IVIS imaging, luciferin substrate was IP injected into each mouse and imaged to obtain radiance flux (photons/s). The red arrow indicates a lung metastasis signal. b For the QPCR calculation of CTC cell numbers from the control and breast-tumor-bearing mice, the human GUS cell numbers were normalized with the corresponding mouse GUS results. Bar errors are represented by three independent experiments, and the p value represents the significance between the control and the tumor-bearing mice. c The upper-panel shows the IVIS Spectrum CT imaging of the subcutaneous tumor model for both breast-tumor-bearing and lung metastasis mice. The mice were positioned in a dorsal view. The lower-panel shows the IVIS images of lung tissues