Fig. 1

Inhibition of MMP-2 and MMP-9 reduced Rb migration. a-b Y79 and Weri-1 cells were added to the upper chamber of an 8 μm polycarbonate membrane coated with basement membrane proteins in serum free media. The lower chamber contained cell culture media with or without MMPI. Six-hours post culture, invasive cells degraded the ECM and were collected, stained and counted. Representative figures are shown in a with a 100× total magnification. Cells were extracted and OD measured in b left for Y79 and right for Weri-1. c Y79 Rb cells were cultured in the presence or absence of MMP-2 or MMP-9 inhibitors for 48-h on poly-L-lysine coated wells with gelatin as substrate. Sterile in-well inserts created a gap of 900 μm. Gap closure was recorded at different time intervals using an Axiovert 40 CFL. Total magnification is 12.5×. Plotted results are in c right. d Weri-1 cells showed increased cell death and detachment from coated surface. For each condition n = 3; gap was measured in 5 different points