Fig. 4

a Representative flow cytometry data showing gating strategy on whole blood samples used to obtain absolute volumetric cell count data. Lymphocytes were identified on the basis of FSC vs. SSC, with CD4⁺ or CD8⁺ T cells subsequently identified from within the CD3⁺ subset. b-c Longitudinal empirical data, linear mixed models and estimated means (left, centre and right-hand panels respectively) for: (b) absolute volumetric cell count data; (c) ratio of CD8⁺ T cells to CD4⁺ T cells (P-values: * <0.05, ** <0.01, *** <0.001, **** < 0.0001)