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Table 1 mRNA panel used to analyse cell mRNA transcripts

From: Single-cell mRNA profiling reveals transcriptional heterogeneity among pancreatic circulating tumour cells

Gene Symbol Gene Name ENSEMBL Gene ID Amplicon length Assay number
Epithelial transcripts
KRT8 Keratin 8 ENSG00000170421 164 Hs01595539_g1
KRT19 Keratin 19 ENSG00000171345 96 AI70M8O (Custom assay)
EPCAM Epithelial cell adhesion molecule ENSG00000119888 64 Hs00158980_m1
CDH1 Cadherin 1; type 1, E-cadherin ENSG00000039068 65 Hs01013953_m1
EMT-associated transcripts
VIM Vimentin ENSG00000026025 73 Hs00185584_m1
CDH2 Cadherin 2; type 1, N-cadherin ENSG00000170558 66 Hs00983056_m1
ZEB1 Zinc finger E-box binding homeobox 1 ENSG00000148516 63 Hs00232783_m1
Cancer stem cell transcripts
CD24 CD24 molecule ENSG00000272398 140 Hs02379687_s1
CD44 CD44 molecule ENSG00000026508 70 Hs01075861_m1
ALDH1A1 Aldehyde dehydrogenase 1; family member A1 ENSG00000165092 61 Hs00946916_m1
Pancreas cancer-associated transcript
SPARC Secreted protein, acidic, cysteine-rich (osteonectin) ENSG00000113140 76 Hs00234160_m1
Reference transcript
HPRT1 Hypoxanthine phosphoribosyltransferase 1 ENSG00000165704 82 Hs02800695_m1
Leucocyte transcript
PTPRC Protein tyrosine phosphatase, receptor type C (CD45) ENSG00000081237 57 Hs04189704_m1