Table 1 Primers used in specific mtDNA amplification
# | Start(bp) | End(bp) | Length(bp) | “Forward” primer (5′–3′) | “Reverse” primer (5′–3′) | Annealing temperature (°C) |
|---|---|---|---|---|---|---|
23 | 15,924 | 201 | 846 | aAACCGGAGACGAAAACCTTTTTC | aCTTTAGTAGGTATGTTCGCCTGT | 51 |
1 | 16,521 | 880 | 928 | aCCATAAAGCCTAAATAGCCCACA | aCCAACCCTGGGGTTAGTATAGCT | 54 |
10 | 6917 | 7671 | 754 | aTGCTCTGAGCCCTAGGATTCATC | aTGAGGGCGTGATCATGAAAGGTG | 55.5 |
16 | 10,852 | 11,566 | 714 | aGCCTAATTATTAGCATCATCCCC | aATGCCTCATAGGGATAGTACAAG | 51 |
22 | 15,169 | 15,993 | 824 | aGAGGGGCCACAGTAATTACAAAC | aTGGGTGCTAATGGTGGAGTTAAA | 51 |