Fig. 1

Immunohistochemical staining of pVHL in tongue tissues. Tissues were stained with hematoxylin and eosin (a, c, e, h, and k), and serial sections were immunohistologically stained for pVHL (b, d, f, g, i, j, l, and m). a and b, normal tongue epithelium; c and d, epithelial dysplasia lesions (arrows). Bars indicate 25 μm. e, f, and g, well-differentiated invasive tongue squamous cell carcinoma; h, i, and j, moderately differentiated invasive tongue squamous cell carcinoma; k, l, and m, poorly differentiated invasive tongue squamous cell carcinoma. Bars indicate 50 μm (e, f, h, i, k, l) and 25 μm (g, j, m)