Fig. 8

PAD inhibition suppresses EGF-induced ductal invasion and elongation in primary mouse mammary organoids. a Isolated mammary gland organoids were cultured in matrigel and were treated with either DMSO (control), EGF (100 ng/ml), EGF (100 ng/ml) + BB-Cl-Amidine (0.5 μM), or EGF (100 ng/ml) + BB-Cl-Amidine (5 μM). Following 6 days of culture, the samples were imaged using Zeiss Axio Observer inverted microscope. b The relative area of invasion and elongation (normalized cell area per field) were measured using image J. Samples were normalized to the control. Data were analyzed using unpaired Student’s t-test and are expressed as the mean ± SEM (*p < 0.01; **p < 0.05; ***P < 0.005)