Fig. 5
From: Role of peptidylarginine deiminase 2 (PAD2) in mammary carcinoma cell migration
Enhanced activity and expression of PAD2 observed in EGF treated MCF10DCIS.com cells. a Scratch assays were performed on serum-deprived MCF10DCIS.com cells treated without (Untreated) or with EGF. MCF10DCIS.com cells were fixed 24 h after wound striking. Cells were visualized using light microcopy to determine the extent of wound closure. Serum-deprived MCF10DCIS.com cells were treated without (Untreated) or with EGF for 1 h prior to immunofluorescent analysis using (b) anti-PAD2 and (c) anti-pan-citrulline antibodies. DAPI was used to visualize the nucleus. Scale bar = 50 μm