Fig. 2

Fluorescence comparison between ab2074 and MSAP-Ac-TZ14011 staining. a EWS cell lines TC32 (white), 6647 (light gray) (high CXCR4 RNA expression), L1062 (dark gray) and A673 (black) (low CXCR4 RNA expression) were similar prepared for the ab2074 and MSAP-Ac-TZ14011 staining following the protocol described by Pelekanos et al. [31]. The ab2074 did not demonstrate a difference in CXCR4 detection between high and low CXCR4 RNA expressing cell lines where MSAP-Ac-TZ14011 did. Representative figures (n = 3) are demonstrated. b MDA-MB-231 X4 and c “Leiden-ALL-HP” cells were prepared and stained similar to the EWS cell lines. In MDA-MB-231 X4 the same difference between ab2074 (green) and MSAP-Ac-TZ14011 (red) staining was demonstrated but in “Leiden-ALL-HP” a higher expression was detected by ab2074 than MSAP-Ac-TZ14011 (both n = 1). The normalized number of counts is demonstrated