Table 1 Sequences for target gene primer for RT-PCR
Gene | Primer sequence 5′-3’ | Tm (°C) | |
|---|---|---|---|
siah2 | F: | GCCCACAAGAGCATTACCAC | 59.80 |
R: | GTTTCTCCAGCACCAGCAT | 57.60 | |
NKX3.1 | F: | GCCAAGAACCTCAAGCTCAC | 59.80 |
R: | TTCTCCAAGTCTCCCAGCTC | 59.80 | |
PMEPA1 | F: | CTCCACCACACACACATCG | 59.70 |
R: | CGCCTTCCTCTCACTCCTCT | 61.90 | |
SLC45A3 | F: | GAGCCGAGACGAAGCAGTT | 59.70 |
R: | GCCAAAGGTTAGCAGGTTGA | 57.80 | |
PSA | F: | TCCTCACAGCTGCCCACT | 60.58 |
R: | ATATCGTAGAGCGGGTGTGG | 59.98 |