Fig. 4

C4-2 (a) and LNCaP (b) cells were incubated for three periods (3, 6 and 9 h) with 5 μM JS-K. RT-PCR was performed to access the influence of JS-K on transcription of specific AR target genes (PSA, NKX3.1, PMEPA1 and SLC45A3). Each assay was performed in triplicate and the expression levels of mRNAs were expressed as 2^-ΔΔCT; c western blotting was performed to detect the influence of JS-K on PSA in C4-2 and LNCaP cells incubated for three periods (3, 6 and 9 h) with 5 μM JS-K. Results are mean ± SD of three different experiments. Single asterisks (*) indicate a significant difference (P < 0.05) and triple asterisks (***) indicates an extremely significant difference (P < 0.001)