Fig. 3

C4-2 and LNCaP cells were incubated for three periods (3, 6 and 9 h) with 5 μM JS-K. a Total ubiquitination protein was diminished by JS-K; b JS-K increased p53, Mdm2 protein levels; c JS-K inhibited the combination of p53 and Mdm2 that were detected by Co-IP; All the IP were used in the input and at least three different experiments were performed; d apoptosis relative proteins (PARP, Bcl-2, Bax, Caspase-9 and Caspase-3) were detected by western blotting. GADPH was set as the loading control