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Fig. 2 | BMC Cancer

Fig. 2

From: JS-K, a nitric oxide pro-drug, regulates growth and apoptosis through the ubiquitin-proteasome pathway in prostate cancer cells

Fig. 2

Caspase 3/7 activity assay. C4-2 cells (a) and LNCaP (b) were treated with indicated concentration of JS-K (5 μM) for three periods (12, 24 and 48 h). The apoptosis of cells was detected by Caspase 3/7 activity assay (Promega) and each assay was performed in triplicate. c Apoptosis induced by JS-K in PCa cells treated with indicated concentration of JS-K (5 μM) for three periods (3, 6 and 9 h) was analyzed by flow cytometry with the Annexin V staining method. Untreated cells were analyzed as control. Data indicated that JS-K could induce apoptosis in PCa cells in a time-dependent manner. Results are mean ± SD of three different experiments. Triple asterisks (***) indicates an extremely significant difference (P < 0.001)

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