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Table 1 Antibodies used in immunolabelling analysis

From: The adaptation of colorectal cancer cells when forming metastases in the liver: expression of associated genes and pathways in a mouse model

Antigen

Species

Dilution

Catalogue

Manufacturer

β-catenin

Rabbit

1:50

14–6765

eBioscience, Frankfurt a.M., Germany

CD44

Rat

1:1000

550,538

BD Pharmingen, Heidelberg, Germany

Ki-67

Rabbit

1:200

275R-14

Cell Marque, California, United States

E-cadherin

Rabbit

1:50

sc-7870

Santa Cruz Biotechnology, Heidelberg, Germany

Vimentin

Rabbit

1:1000

ab92547

Abcam, Cambridge, UK

Anti-rat biotinylated

Donkey

1:200

RPN1004

GE Healthcare, Freiburg, Germany

Avidin HRP

 

1:400

18–4100-94

eBioscience, Frankfurt a.M., Germany

HRP Labelled anti-rabbit

Goat

Ready to use

K4002

Dako, Hamburg, Germany