Fig. 3

TanIIA decreased CXCL12-induced A375 cell migration. To test the effects of Tan II A on melanoma cell migration, A375 cells were incubated with TanIIA (0, 1, 2, or 4 μg/mL) for 48 h in 12-well plate. The sterilized P200 pipette tip was used to create a straight line scratch on the monolayer cells. The cells in each well were treated with 10 ng/ml chemokine CXCL12 for 48 h. We monitored the cells migration to the scratch area at 0, 24 and 48 h. For each image, the interval distance in micrometer (μM) between one sides of scratch to the other was measured. a. The images of A375 cells in 12- well with one scratch line under a microscope at 0, 24, or 48 h post scratch (200 X amplification). b. Tan II A significantly increased scratch clear length in a dose dependent manner. Each treatment was repeated three times (n = 3). *P < 0.05, ** P < 0.01, and ***P < 0.001 for comparison of indicated treatments to the negative control (0 μg/mL) at 0, 24 or 48 h post scratch respectively