Fig. 2

TAS-115 inhibits the growth of SS cells by inducing G0/G1 cell cycle arrest and apoptosis. a Yamato-SS, SYO-1 and HS-SY-II cells (3 × 10³) were treated with TAS-115 in a concentration range of 0 to 10 μM for 72 h. Relative cell proliferation rates were determined using a WST-1 assay. Bars represent the SD. b Calculated IC₅₀ values of each cell line. c The effect of TAS-115 on the cell cycle. Yamato-SS, SYO-1 and HS-SY-II cells were treated with control (0.1% DMSO) or 0.1–10-μM TAS-115 for 24 h. After treatment, the cells were stained with PI solution for flow cytometric analysis. d The effect of TAS-115 on PARP cleavage in Yamato-SS, SYO-1 and HS-SY-II cells. Cells were treated control (0.1% DMSO) or 0.001–10 μM of TAS-115 for 24 h