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Fig. 7 | BMC Cancer

Fig. 7

From: Linking hypoxia, DNA damage and proliferation in multicellular tumor spheroids

Fig. 7

ATM or ATR inhibition attenuates the severe hypoxia-induced increase in γ-H2AX levels in spheroids. a A673 spheroids ~400 μm in diameter were maintained for 12 h in either 20% O2 or 1% O2 in vehicle, 1% O2 in KU55933 or 1% O2 in VE-821. The effect of ATM inhibition (KU55933) or ATR inhibition (VE-821) on γ-H2AX distribution (red, with blue Hoechst 33,342 counter-stain) is shown. b Percentage of γ-H2AX-positive cells in spheroids was quantitated. Bar graph shows the mean and standard deviation from >14 spheroids and at least 3 independent experiments. One way ANOVA with Dunnett’s post-test. ***P < .001, **P < .01, ns P > .05

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