Fig. 4
From: Linking hypoxia, DNA damage and proliferation in multicellular tumor spheroids
Distribution of γ-H2AX-positive cells in A673 spheroids. a γ-H2AX in A673 spheroids was used as a marker of DNA damage repair signaling. Immunostaining of representative spheroids <500 μm, 550–650 μm and >750 μm in diameter is shown. γ-H2AX in red, with blue Hoechst 33,342 counter-stain. b γ-H2AX co-staining with Ki-67 in spheroids approximately 570–650 μm in diameter shows activated DDR in proliferative cells within the hypoxic zone. The outer dotted line indicates where Ki-67-positive nuclei decrease in number, inner dotted line marks boundary of viable cells