Fig. 1
From: Linking hypoxia, DNA damage and proliferation in multicellular tumor spheroids
Growth characteristics of A673 and LLC spheroids. a Growth curves for spheroids generated with A673 (red) and LLC (black) cells. The mean diameter and standard deviation from >8 spheroids are plotted over time, from initial formation to final size before spheroid collapse. b H&E staining of spheroid cryo-sections reveals the onset of necrosis in spheroids larger than 550 μm. The three sections shown come from spheroids of <500 μm, 550–650 μm and >750 μm in diameter. Shrinkage during processing as well as location of section within spheroids account for the smaller diameter in the final images shown