Fig. 1

Summary of the ASONECell Picking System. a Fluorescently labeled cells are loaded in a microchamber array and sorted by the machine. b Scatter plot of mean fluorescence intensities for CK-PE (x-axis) and CD45-APC (y-axis) staining. c Bright-field, PE, and APC channel images of peripheral blood mononuclear cells (PBMCs) and circulating tumor cells (CTCs). CTCs can be distinguished from contaminated leukocytes by combining the fluorescence filters. d Cells marked with a red circle are automatically collected with a glass capillary