Fig. 2From: Gamma-glutamyltransferase activity in exosomes as a potential marker for prostate cancerGGT1 expression in exosomes isolated from PC cells by immunocapture. The culture medium of LNCaP, C4, C4–2 and C4–2B cells (30 mL) were incubated with magnetic beads (1 mg) conjugated with anti-CD9 (a) or -PSMA (b) antibody at 4 °C for 90 min. Whole immunocaptured exosomes were subjected to Western blot analysis for GGT1 large and small subunits, CD9, Alix and PSMABack to article page