Fig. 4

Functions of miR-148a in GLA-induced anti-angiogenesis. Cells were treated as described in Fig. 3, and the conditioned media were collected. a The ELISA was used to detect VEGF secretion (mean ± SD, n = 3). The previous media with anti-miRNAs and GLA were removed, and the cells were washed with 1× PBS and replaced with fresh media with 1% serum for 24 h, and the conditioned media was collected. b HUVECs were exposed to the conditioned mediums collected as described in (a) for 6 h. c Quantitative analyses of the tube numbers, the total number of formed tube branches in each well was counted under the light microscope (mean ± SD, n = 5). ^* P < 0.05 and ^** P < 0.01 compared with the anti-miR-negative control. ^# P < 0.05 compared with the cells treated with GLA and anti-miR-negative control