Skip to main content
Fig. 2 | BMC Cancer

Fig. 2

From: The repressive effect of miR-148a on Wnt/β-catenin signaling involved in Glabridin-induced anti-angiogenesis in human breast cancer cells

Fig. 2

GLA blocks Wnt/β-catenin signaling in MDA-MB-231 cells. MDA-MB-231 cells were exposed to 10 or 20 μM GLA for 48 h. a Western blot analyses relative protein levels of Wnt1, non-phospho (active) β-catenin, and β-catenin. b Expression of Wnt1 and β-catenin (mRNAs) were analyzed by the quantitative real-time polymerase chain reaction (qRT-PCR) (mean ± SD, n = 3). c Immunofluorescence assay analyses the nuclear translocation of β-catenin. d Expression of TCF/LEF4 (mRNAs) analyzed by qRT-PCR (mean ± SD, n = 3). ** P < 0.01 and *** P < 0.001 compared with the control cells

Back to article page