Table 1 Primer sequences of real-time PCR
From: MicroRNA-30a regulates cell proliferation and tumor growth of colorectal cancer by targeting CD73
Gene | Sequence (5′ - 3′) | |
|---|---|---|
CD73 | Forward Primer | ATTGCAAAGTGGTTCAAAGTCA |
Reverse Primer | ACACTTGGCCAGTAAAATAGGG | |
GAPDH | Forward Primer | GAGTCAACGGATTTGGTCGT |
Reverse Primer | GACAAGCTTCCCGTTCTCAG | |
miR-30a | Forward Primer | GCGTGTAAACATCCTCGAC |
Reverse Primer | GTGCAGGGTCCGAGGT | |
U6 | Forward Primer | CTCGCTTCGGCAGCACA |
Reverse Primer | AACGCTTCACGAATTTGCGT |