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Table 1 Immunohistochemistry profile at the time of first evaluation

From: Alpha–fetoprotein elevation in NUT midline carcinoma: a case report

Weakly positive Clues to diagnosis (if positive) Negative Clues to diagnosis (if positive)
AE1-AE3 cytokeratin pool Carcinomas, GCT (rare), thymoma, mesenchymal tumors PLAP Seminoma
TTF-1 Lung, thyroid cancer AFP Non-seminoma GCT, HCC, pancreas carcinoma
p63 Basal/squamous carcinoma, mesenchymal tumors, lymphomas, adenoid cystic carcinoma, salivary gland tumors, thymoma, thymic carcinoma, breast, urothelial carcinoma Beta-HCG Non-seminoma GCT
Synaptophysin Neuroendocrine neoplasms, adrenal gland carcinoma, mesenchymal tumors CD30 Lymphomas (HL and NHL), non-seminoma GCT,
Chromogranin A Neuroendocrine tumors CD3 T-cell lymphomas
CD34 Hematologic malignances, mesenchymal tumors, papillary thyroid carcinoma, thymoma CD20 B-cell lymphomas
Focally positive   CD117 Mesenchymal tumors, GCT, SCLC, leukemias, lymphomas, melanoma
EMA adenocarcinoma, renal cell carcinoma, choriocarcinoma, mesenchymal tumors, mesothelioma, lymphoma, thymic carcinoma Melan-A Melanoma, mesenchymal tumors, adrenal cortical tumors, sex-cord stromal tumors
Cytokeratin 7 Lung cancer and other carcinomas, GCT (rare), mesenchymal tumors S-100 Melanoma, mesenchymal tumors, adenoid cystic carcinoma, sex-cord stromal tumors
   ALK Lymphomas, NSCLC, mesenchymal tumors, RCC (rare)
   PAX8 Thymic carcinoma, thymomas, thyroid anaplastic carcinoma, RCC, neuroendocrine neoplasms, seminoma
   Desmin Mesenchymal tumors
  1. TTF-1 thyroid transcription factor-1, EMA epithelial membrane antigen, PLAP placental alkaline phosphatase, AFP alpha-fetoprotein, beta-HCG human chorionic gonadotropin, ALK anaplastic lymphoma kinase, GCT germ cell tumor, HCC hepatocellular carcinoma, HL Hodgkin’s lymphoma, NHL Non-Hodgkin lymphoma, RCC renal cell carcinoma, SCLC small cell lung cancer, NSCLC non-small cell lung cancer