Fig. 1

Low-dose of arsenic promotes cell proliferation. a E7 cells were treated with indicated concentration of NaAsO2 for one week and the percentage of cell population was detected by flow cytometry. b E7 cells were treated with or without arsenic (1 μM) for 1 week. BrdU (10 μM) was added into the cultured medium for 1 h before fixing the cells by 4% paraformaldehyde. BrdU positive cells (green color) were stained by BrdU antibody conjugated with FITC (left panel). The quantification of BrdU positive-cells was shown in the right panel. Data were represented as mean ± S.D. of 2 independent experiments. Student’s t test was used, *p < 0.05, **p < 0.01, ***p < 0.001 compared with non-arsenic treated cells (0 μM)