Fig. 6

THF inhibited syngeneic tumor growth in vivo. Mice were injected s.c. with 1.5 × 10⁶ 4 T1 cells and then treated daily with THF at 80 or 160 mg/kg by gavage for 21 consecutive days. a The anatomic morphologic observation of syngeneic tumors in control and treatment groups. Tumor volume (b) and tumor weight (c) was calculated at indicated time points after treatment. d Representative Ki67 IHC staining of control or treated tumor tissues from mice (Scale bar =25 μm). e Quantitative analysis of Ki67 staining corresponding to the images in d. Data represent the mean ± S.D. of three independent experiments. (*P < 0.05, **P < 0.01, ***P < 0.001 vs control)