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Fig. 2 | BMC Cancer

Fig. 2

From: Hypermethylation of CDKN2A exon 2 in tumor, tumor-adjacent and tumor-distant tissues from breast cancer patients

Fig. 2

Analysis of DNA standards by MS-HRM (ad) and PSQ (e). a, c: Normalized melting curves for ESR1 (a) and CDKN2A (exon 2) (c) obtained by analyzing mixtures of unmethylated and fully methylated control DNA. Replicate view of duplicate measurements carried out on one day. b, d: Calibration curves for ESR1 (b) and CDKN2A (exon 2) (d) obtained by repeatedly analyzing control DNA standards (ESR1: n = 6; CDKN2A (exon 2): n = 8). e: Representative pyrogram for ESR2 obtained by analyzing methylated control DNA. Peaks highlighted in blue indicate the methylation status of the CpGs in the sequence to analyze. The position highlighted in orange serves as control for complete bisulfite conversion

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