Fig. 5
Inhibition of BxPC3 chemotaxis towards LPA by Ki16425. a BxPC3 chemotaxis towards 1 μM LPA with various concentrations of Ki16425. Cells were pre-incubated with Ki16425 for 24 h and the chemotaxis assay was performed using TAXIScan. Data are representative of 3 independent experiments. b Box-plots of the directionality and the velocity in BxPC3 migration towards LPA with Ki16425. The graph on the left indicates directionality and that on the right indicates velocity. The half maximal inhibitory concentration (IC50) values are also shown. Statistical analysis was done by the Kruskal-Wallis Test (Non-parametric ANOVA) followed by the Dunn’s Multiple Comparisons Test. ***p < 0.0001 (vs. data with 1 μM LPA and without Ki16425). Data are representative of 3 independent experiments. c Inhibition of phosphorylation of Akt or p44/42MAPK by Ki16425 in BxPC3 and PANC-1 cell lines, as indicated. Cell lysates taken after LPA stimulation at each time point were analyzed by SDS-PAGE and western blotting. Anti-β-actin antibody was used as the internal control. Arrows indicate the specific band for each antibody. Data are representative of 3 independent experiments