Fig. 4

Phosphorylation of receptors or signaling molecules. a and c Images of phosphorylation of receptors in BxPC3 (a) or in PANC-1 (c) cell lines detected by Antibody Array. Data are representative of 3 independent experiments. b and d The quantitation of phosphorylation by measuring density of Antibody Array with BxPC3 data (b) or with PANC-1 data (d). e Phosphorylation of Akt or p44/42MAPK in BxPC3 and PANC-1 cell lines, as indicated. Cell lysates taken after LPA stimulation at each time point were analyzed by SDS-PAGE and western blotting. Anti-β-actin antibody was used as the internal control. Arrows indicate the specific band for each antibody. Data are representative of 2 independent experiments