Fig. 6

miR-181a is regulated by lncRNA XIST. a Diagram of the three miR-181a putative binding sites and corresponding mutant sites in XIST mRNA sequences. b Effects of miR-181a on the expression of reporter genes containing wt-XIST or mut-XIST sequences. ** vs Blank, p < 0.01. c Relative expression of XIST in HCC cells and the normal liver cells. ** vs L-02, p < 0.01. d Relative expression of XIST in HCCLM3 cells after transfection with miR-181a inhibitor. ** vs Blank, p < 0.01. e Relative expression of miR-181a in Huh7 cells after transfection with si-XIST or si-Scramble. ** vs Blank, p < 0.01. f Relative expression of miR-181a in HCC clinical samples and the normal. g The relationship between relative expression of XIST and miR-181a. lncRNA, long non-coding RNA; mut, mutant; wt, wild-type; HCC, hepatocellular carcinoma