Fig. 6
Effects of NAC on 2250 induced cell death in cell line AsPC-1 measured by FACS analysis. AsPC-1 cells were incubated with either 2250 (500, 1000 μmol/l), NAC (5 mmol/l) or the combination of both agents (2250 500, 1000 μmol/l + NAC 5 mmol/l) and ddH2O as control for 24 h. The percentage of viable (a), apoptotic (b) and necrotic (c) cells were determined by FACS analysis. Values are means ± SEM of 4–6 replicates of three independent experiments with consecutive passages. Asterisk symbols indicate differences between control, which was adjusted to 100% and 2250 treatment. *** p ≤ 0.001, ** p ≤ 0.01, * p ≤ 0.05, n.s. p > 0.05 (one-way ANOVA followed by Tukey’s post-hoc test)