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Fig. 5 | BMC Cancer

Fig. 5

From: Innovative substance 2250 as a highly promising anti-neoplastic agent in malignant pancreatic carcinoma - in vitro and in vivo

Fig. 5

Effects of NAC on 2250 induced cell death in different malignant cell lines measured by MTT (a-e); impact of 2250 on the cellular level of ROS (f). AsPC-1 (a), BxPC-3 (b), MiaPaca-2 (c), Panc-1 (d) and Panc TuI (e) cells were incubated with either 2250 (500, 1000 μmol/l), NAC (5 mmol/l) or the combination of both agents (2250 500, 1000 μmol/l + NAC 5 mmol/l) and ddH2O as control for 24 h and submitted to a MTT-assay. The level of ROS was analyzed in untreated compared to 2250 treated cells, additional NAC treatment served as a neg. Control (f). Values are means ± SEM of 6 replicates of three independent experiments with consecutive passages. Asterisk symbols indicate differences between control, which was adjusted to 100% and 2250 treatment. *** p ≤ 0.001, ** p ≤ 0.01, * p ≤ 0.05, (one-way ANOVA followed by Tukey’s post-hoc test)

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