Fig. 4

miR-30d acts as an oncomir in CSCCs. a Expression levels of miR-30d were examined by real-time PCR; b After treatment of miR-30d inhibitor, mimic and control strand, the expression levels of miR-30d were examined by real-time PCR. The relative expression of miR-30d is illustrated as a ratio to control (U6); c WST-1 (Roche) assay measuring the activity of mitochondrial dehydrogenases was performed following the manufacturer’s instruction at 0-, 1-, 2-, 3-, 4- day time points. The results were obtained from three independent experiments. Error bars represent the standard deviation of the mean; d Cell migration was determined using a transwell assay as described in the Materials and methods section. Microscopic image of migrated HeLa and SiHa cells with indicated treatments. Diagrams of migrating cells from the different transfectants are shown, which are from more than three independent experiments.*p < 0.05 versus control. e-f Tumor growth indicates the stable inhibition of miR-30d expression in CSCC cell lines when subcutaneously injected into the right (inhibitor of miR-30d) and left (control) flanks of male nude mice (n = 15). Tumor growth was followed for 42 after tumor cell injection. The dotted line represents the tumor mass. g Expression of miR-30d extracted from xenografts using qPCR. The expression level of miR-30d was normalized to the expression state of U6. Values represent the means, and the error bars represent the SD. *p < 0.05 according to the paired t-test