Fig. 2

Confirmation of miRNA transfection and anti-miR functionality. Cy3-conjugated scrambled anti-miRNA molecules were transfected into (a) MEN-117, (b) MEN-117 negative control, (c) MEN-141, and (d) MEN-141 negative control for direct observation of cellular uptake. Observation was made 8 h after transfection. The red spots represent Cy3-labeled anti-miRNA molecules and the nucleus is stained with DAPI (blue). No Cy3 labeled anti-miRNA mimics were transfected to negative controls but only DAPI. Scale bars indicate 12 μm length. Anti-miRNA molecules were transfected into MEN-117 and MEN-141 cells. The ability of anti-miRNA molecules to decrease corresponding miRNA levels: (e) The decreased level of miR-29c-3p by anti-miR-29c-3p. f The decreased level of miR-4492 by anti-miR-4492. *p < 0.05; **p < 0.01