Fig. 4
From: Morphological and phenotypical features of ovarian metastases in breast cancer patients
Detection of ovarian metastases by a combination of markers. Representative image of a lobular ovarian metastasis stained with DAPI counterstain and triple immunofluorescence for EpCAM (a), EMA (b), Her2/neu (c), and the three stainings combined (d). The solid arrow indicates tumor cells that are positive for EpCAM, but negative for EMA and Her2/neu. The dashed arrow indicates tumor cells that are positive for Her2/neu, but negative for EpCAM and EMA. Scale bars represent 100 μm. EpCAM, epithelial cell adhesion molecule; EMA, epithelial membrane antigen; Her2/neu, human epidermal growth receptor type 2