Fig. 2

Proliferation of TUHR14TKB cells during passage. a The doubling times of TUHR14TKB cells and its subclones were subjected to MTS assay. Low and high passages are defined as TUHR14TKB cells 7–9 and 19–23 passages, respectively. “Subclones 1, 2, and 3” represents subclones from low-passage TUHR14TKB cells. The assay was repeated three to five times, and the data represent the average value and standard deviation (error bars). b The stages of the cell cycle of low- and high-passage TUHR14TKB cells and the subclones were determined using flow cytometry