Table 1 Primer sequence and qPCR setting
From: u-PAR expression in cancer associated fibroblast: new acquisitions in multiple myeloma progression
Gene | Forward primer | Reverse primer |
|---|---|---|
u-PA | 5′-AGTGTCAGCAGCCCCACT-3′ | 5′-CCCCCTGAGTCTCCCTGG-3’ |
u-PAR | 5′-GCCCAATCCTGGAGCTTGA-3’ | 5′-TCCCCTTGCAGCTGTAACACT-3’ |
PAI-1 | 5′-CTCCTGGTTCTGCCCAAGTT-3’ | 5′-GAGAGGCTCTTGGTCTGAAAG-3’ |
MMP-2 | 5′-AGCACCGCGACAAGAAGTAT-3’ | 5′-ATTTGTTGCCCAGGAAAGTG-3’ |
18S rRNA | 5′-CGGCTACCACATCCAAGGAA-3’ | 5′-GCTGGAATTACCGCGGCT-3’ |
α-SMA | 5′-CTGTTCCAGCCATCCTTCAT-3’ | 5′-CCGTGATCTCCTTCTGCATT-3’ |
Vimentin | 5′-TGTCCAAATCGATGTGGATGTTTC-3’ | 5′-TTGTACCATTCTTCTGCCTCCTG-3’ |